Total RNA from frozen tissues was extracted using TRIZOL reagent. RNA was extracted from formalin-fixed, paraffin-embedded tissues by digestion with proteinase K before the acid-phenol:chloroform ...

As technologies and methodologies advance, so do the potential applications for biospecimens. In this article, we explore seven uses of FFPE, ranging from the common to the cutting-edge.

Global micro-RNA (miR) profiling of human malignancies is increasingly performed, but to date, the majority of such analyses have used frozen tissues. However, formalin fixation is the standard and ...

An invaluable source of RNA, DNA, and chromatin from historical and clinical samples is formalin fixed paraffin embedded (FFPE) tissue. Globally, tissue banks, hospitals, and laboratories are ...

Circulating tumor cells as a surrogate marker for determining response to chemotherapy in Japanese patients with metastatic colorectal cancer. Background: miRNAs have been shown to be involved in ...

Formalin-fixed paraffin-embedded (FFPE) tissues are the most widely used clinical sample types in histology and molecular diagnosis. However, due to RNA degradation and protein crosslinking, FFPE ...

The relatively new field of onco-metabolomics attempts to identify relationships between various cancer phenotypes and global metabolite content. Previous metabolomics studies utilized either nuclear ...

A single-arm trial of panitumumab in cetuximab refractory KRAS wild-type colorectal cancer. Background: Genomic profiling has improved our understanding of the underlying biology of tumors, accuracy ...