Spectrophotometry and the Beer–Lambert Law
10:23
Khan AcademySal Khan
Spectrophotometry and the Beer–Lambert Law
Spectrophotometry is a technique that uses light absorption to measure the concentration of an analyte in solution. The amount of light absorbed by a solution is related to the analyte concentration by the Beer–Lambert law, which is expressed as follows: A = εbc, where ε is the molar absorptivity of the analyte, b is the path length (the ...
Feb 20, 2011
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